Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novel ornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl(-1) with NAA ( 0.0, 0.5, 1.0 mgl(-1)) and BA (0.0, 0.5, 1.0, 2.0 mgl(-1)) with IAA ( 0.0, 0.5, 1.0, mgl(-1)) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl(-1) BA and 1.0 mgl(-1) NAA (70%) followed by 1.0 mgl(-1) BA and 0.5 mgl(-1) NAA (50%), 1.0 mgl(-1) BA and 1.0 mgl(-1) NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl(-1) BA and 0.5 mgl(-1) IAA (43%) followed by 0.5 mgl(-1) BA and 1.0 mgl(-1) IAA (23%)

Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novelornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl-¹ with NAA (0.0, 0.5, 1.0 mgl-¹) and BA (0.0, 0.5, 1.0, 2.0 mgl-¹) with IAA (0.0, 0.5, 1.0, mgl-¹) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl-¹ BA and 1.0 mgl-¹ NAA (70%) followed by 1.0 mgl-¹ BA and 0.5 mgl-¹ NAA (50%), 1.0 mgl-¹ BA and 1.0 mgl-¹ NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl-¹ BA and 0.5 mgl-¹ IAA (43%) followed by 0.5 mgl-¹ BA and 1.0 mgl-¹ IAA (23%)

Agrobacterium-mediated transformation of kabocha squash (Cucurbita moschata Duch) induced by wounding with aluminum borate whiskers

An efficient genetic transformation method for kabocha squash (Cucurbita moschata Duch cv. Heiankogiku) was established by wounding cotyledonary node explants with aluminum borate whiskers prior to inoculation with Agrobacterium. Adventitious shoots were induced from only the proximal regions of the cotyledonary nodes and were most efficiently induced on Murashige–Skoog agar medium with 1 mg/L benzyladenine. Vortexing with 1% (w/v) aluminum borate whiskers significantly increased Agrobacterium infection efficiency in the proximal region of the explants. Transgenic plants were screened at the T0 generation by sGFP fluorescence, genomic PCR, and Southern blot analyses. These transgenic plants grew normally and T1 seeds were obtained. We confirmed stable integration of the transgene and its inheritance in T1 generation plants by sGFP fluorescence and genomic PCR analyses. The average transgenic efficiency for producing kabocha squashes with our method was about 2.7%, a value sufficient for practical use

α-thalassaemia

Alpha-thalassaemia is inherited as an autosomal recessive disorder characterised by a microcytic hypochromic anaemia, and a clinical phenotype varying from almost asymptomatic to a lethal haemolytic anaemia

Impact of tuber location on morphological characteristics of Cyclamen

In Cyclamen genus, there are differences at some degree of morphological characters among closely related wild species with disjunct distributions. Two hundred and fiveTurkish landraces of cyclamen coming from different areas containing different temperature, altitudes, soil and vegetation, relative humidity and light intensity were evaluated for 12 quantitative morphological traits of flowers and leaves such as petal length, petal width, flower number/plant, pedicel length, basal corolla ring diameter, pistil length, stamen length, leaf number/plant, petiole length, lamina length, lamina width, plant growth habit, in Cyclamen mirabile, Cyclamen alpinum and Cyclamen hederifolium. According to the location of species, all three species showed morphological differentiation in two traits (pedicel length and petiole length). C. mirabile showed high levels of morphological differentiation from other species (in eight traits out of 12, as 66,7%), followed by C. hederifolium (in six traits out of 12, as 50%) and C. alpinum (in four traits out of 12, as 33,3%). © 2017, Pakistan Botanical Society. All rights reserved

Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novelornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl-¹ with NAA (0.0, 0.5, 1.0 mgl-¹) and BA (0.0, 0.5, 1.0, 2.0 mgl-¹) with IAA (0.0, 0.5, 1.0, mgl-¹) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl-¹ BA and 1.0 mgl-¹ NAA (70%) followed by 1.0 mgl-¹ BA and 0.5 mgl-¹ NAA (50%), 1.0 mgl-¹ BA and 1.0 mgl-¹ NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl-¹ BA and 0.5 mgl-¹ IAA (43%) followed by 0.5 mgl-¹ BA and 1.0 mgl-¹ IAA (23%)

Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novel ornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl-1 with NAA (0.0, 0.5, 1.0 mgl-1) and BA (0.0, 0.5, 1.0, 2.0 mgl-1) with IAA (0.0, 0.5, 1.0, mgl-1) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl-1 BA and 1.0 mgl-1 NAA (70%) followed by 1.0 mgl-1 BA and 0.5 mgl-1 NAA (50%), 1.0 mgl-1 BA and 1.0 mgl-1 NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl-1 BA and 0.5 mgl-1 IAA (43%) followed by 0.5 mgl-1 BA and 1.0 mgl-1 IAA (23%). © 2009 Academic Journals

Morphological characterization of Cyclamen sp. grown naturally in Turkey: Part II [Morfologiczna charakterystyka Cyclamen sp. rosnacych w Turcji w warunkach naturalnych: Czesc II]

The morphology of 279 accessions of Cyclamen sp. growing naturally in Tur-key, namely C. alpinum (syn. C. trochopteranthum), C. graecum, C. hederifolium (syn. C. neapolitanum) and C. mirabile, was characterized. Plants with intact tubers were collected from locations in Antalya, Isparta, Aydın, Muğla, İzmir and Denizli, determined by GPS, where they grow naturally in spring and autumn. The morphology of the four Cyclamen species was characterized using one year old regenerated plants based on 27 morphological traits (13 flower, 11 leaf, 2 plant, 1 tuber). There were distinct differ-ences among these accessions related to petal colour, pedicel length, leaf length and width, leaf shape, and tuber diameter. Even though principle component analysis con-firmed the grouping of characters into species-specific clusters, a wider range of morpho-logical data as well as molecular data are needed for more reliable conclusions to be drawn about the classification of these Cyclamen species. © by Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie, Lublin 2016

The effects of different hormones on regeneration of gazania (Gazania rigens)

Recent advances in the modern techniques of propagation could help growers to meet the demand of the ornamental industry. New ornamental plant varieties are being created by breeders in response to consumer demand for new products. In this study, different concentrations of BA (0.0, 0.2, 0.5, 1.0 mg/l) and IAA (0.0, 0.5, 1.0 mg/l) were investigated to optimize regeneration of Gazania rigens. The best callus formation was found from the media containing 0.2 mg/l BA-1.0 mg/l IAA (100 %), 0.5 mg/l BA-1.0 mg/l IAA (98 %), 0.5 mg/l BA-0.5 mg/l IAA (97 %), 1 mg/l BA -1 mg/l IAA (97 %), 1.0 mg/l BA-0.5 mg/l IAA (98 %), 0.5 mg/l BA (97 %). The most promising regeneration and growth were obtained from the media with 0.2 mg/l BA-1.0 mg/l IAA (30 %), 1.0 mg/l BA-0.5 mg/l IAA (22 %) and 1.0 mg/l BA (18 %). The regenerated plantlets were rooted on the media containing 0.5 and 1.0 mg/l IAA. The best rooting percentage was observed on the media containing 1.0 mg/l IAA (75 %). Copyright © 2009 University of Bucharest

Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers

PubMed ID: 27604272The genus Cyclamen (family Myrsinaceae) contains about 20 species, most of which occur in the Mediterranean region. Turkey has critically important Cyclamen genetic resources. Molecular characterization of plant materials collected from different regions of Turkey in which Cyclamen species grow naturally, namely Adana, Antalya, Aydın, Muğla, İzmir, Denizli, Kahramanmaraş, Osmaniye, Eskişehir, Trabzon, and Rize provinces, was performed using RAPD and SRAP markers. DNA was successfully amplified by 30 RAPD primers and 14 SRAP primer pairs. Among the 470 bands generated by the RAPD primers, 467 were polymorphic. The number of bands detected by a single primer set ranged from 11 to 22 (average of 15.6). The percentage polymorphism was 99.3 % based on the RAPD data. In the SRAP analysis, a total of 216 bands were generated, showing 100 % polymorphism. The number of bands detected by a single primer set ranged from 9 to 22 (average of 15.4). All data were scored and UPGMA dendrograms were constructed with similar results in both marker systems, i.e., different species from nine provinces of Turkey were separated from each other in the dendrograms with the same species being clustered together. © 2016 Springer Science+Business Media New Yor